Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 100-102.doi: 10.3969/j.issn.1673-8225.2011. 01.022

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Effects of methylene blue photochemical virus inactivation on plasma components

Jiao Hong-liang1, 2, Guan Fang-xia3, Yang Bo1, Li Jian-bin2, Shan Hong2, Du Ying4, Hu Xiang5   

  1. 1Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    2Henan Province Red Cross Blood Center, Zhengzhou  450014, Henan Province, China
    3Department of Biomedical Engineering, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    4Department of Microorganism and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    5Beike Bio-Technology, Shenzhen  518000, Guangdong Province, China
  • Received:2010-10-08 Revised:2010-11-12 Online:2011-01-01 Published:2011-01-01
  • Contact: Yang Bo, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Jiao Hong-liang☆, Studying for doctorate, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Henan Province Red Cross Blood Center, Zhengzhou 450014, Henan Province, China jhl787878@126.com
  • Supported by:

    the Key Subject Construction Program of Third-Stage 211 Engineering of Zhengzhou University, the Medical Science and Technology Innovation Talent Engineering Program of Henan Province, No. 2005018

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Abstract:

BACKGROUND: Virus inactivation to blood is one of blood transfusion safety measures. The effect of virus inactivation on human plasma by methylene blue/light has been confirmed, but the report concerning virus inactivation to plasma on plasma components is very few.
OBJECTIVE: To observe the effects of methylene blue photochemical virus inactivation to blood plasma on blood components’ structure and function.
METHODS: A total of 40 blood samples were randomly selected. Fresh plasma from 400 mL whole blood within 6 hours was prepared and the quality was weighed for reserve samples, and then the filter with methylene blue virus inactivation was sterilely connected. The final concentration of methylene blue was 0.9-1.3 μmol/L. Plasma added to methylene blue virus inactivation into 4 ℃ was put on a shelf box, with beat frequency of 60 times/minutes. 32 000-38 000 Lx intensity of 4 ℃ visible light was used to irradiate for 35 minutes. The residual methylene blue and white blood cells were filtered out from the plasma after illumination by the virus inactivated filters, mixed and remained sample 10 mL, and immediately placed in -80 ℃ refrigerator. The amount of plasma samples before and after exposure, methylene blue concentration, F Ⅷ: C, F Ⅴ: C, VWF, Fib Content was detected.
RESULTS AND CONCLUSION: After virus inactivated plasma, the recovery rate of plasma volume, F Ⅷ: C, F Ⅴ: C, VWF, Fib were (96.39 ± 1.73)%, (82.55 ± 9.25)%, (81.03 ± 15.27)%, ( 93.25 ± 6.17)%, (81.61 ± 14.25)%. The effect of methylene blue photochemical virus inactivation in plasma on most components of the plasma is not obvious, and meets the clinical demand for safe blood transfusion.

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